Research-grade peptides are defined not by marketing claims, but by how they are verified, analyzed, and documented before use in laboratory settings. Analytical validation methods such as High-Performance Liquid Chromatography (HPLC) and third-party Certificates of Analysis (COAs) are essential tools for confirming identity, purity, and batch consistency.
Why Verification Matters in Peptide Research
In peptide research, even small variations in purity or composition can influence experimental reproducibility. For this reason, reputable laboratories rely on standardized analytical techniques and documented verification protocols rather than unverified supplier claims.
Verification helps researchers confirm:
- Compound identity and molecular integrity
- Measured purity levels
- Batch-to-batch consistency
- Traceability of production and testing
High-Performance Liquid Chromatography (HPLC)
High-Performance Liquid Chromatography (HPLC) is one of the most widely used analytical methods for peptide verification. HPLC separates compounds based on their interaction with a stationary phase and a mobile solvent, producing a chromatogram that allows researchers to assess composition and purity.
In peptide analysis, HPLC is commonly used to:
- Confirm peptide identity by retention time
- Estimate purity as a percentage of the total sample
- Detect secondary peaks or impurities
HPLC results are typically presented as chromatograms within a Certificate of Analysis.
Understanding Purity Percentages
Purity values reported for research-grade peptides usually reflect the proportion of the target compound detected relative to other measurable components. While higher purity is often preferred for controlled experiments, acceptable purity thresholds may vary depending on the research model and methodology.
It is important to note that purity values:
- Are analytical measurements, not performance guarantees
- Do not imply biological outcomes
- Should be interpreted within experimental context
Certificates of Analysis (COAs)
A Certificate of Analysis (COA) is a formal laboratory document summarizing analytical results for a specific batch of material. COAs typically include:
- Compound name and batch number
- Analytical methods used (e.g., HPLC)
- Reported purity values
- Date of analysis and testing laboratory
COAs allow researchers to independently review analytical data prior to use and are a standard requirement in professional research environments.
Example: BPC-157 Certificate of Analysis
As part of our internal quality review process, Poly Biotech has received a third-party Certificate of Analysis for a recent batch of BPC-157. The COA includes documented HPLC results and reported purity values for that specific batch.
This documentation is provided for research transparency and analytical reference only. The presence of a COA does not imply clinical suitability, therapeutic use, or biological outcomes.
Verification Is an Ongoing Process
Analytical verification is not a one-time event. Responsible research suppliers evaluate each batch individually, maintain documentation, and review analytical data as part of ongoing quality oversight.
For researchers, reviewing COAs and understanding analytical methods is a critical step in selecting materials appropriate for controlled laboratory studies.
