5AM (5-Amino-1MQ) - Complete Research Guide

5-Amino-1MQ, commonly shortened to 5AM, is a small molecule studied for its effects on fat metabolism, insulin sensitivity, and energy efficiency at the cellular level. It works by inhibiting the enzyme NNMT (Nicotinamide-N-methyltransferase), which regulates NAD+ levels and plays a role in fat storage and energy balance.

Quick Facts

CAS: 163889-63-4
Formula: C₁₂H₁₃N₅
Molecular Weight: ~227 g/mol
Storage: Store lyophilized powder at -20 °C. Once reconstituted, refrigerate at 2–8 °C and use within 30 days.

How Does 5AM Work?

5AM is best known as an NNMT inhibitor. NNMT is an enzyme involved in methylation reactions that affect cellular energy balance. When NNMT activity is high, NAD+ levels are depleted, leading to reduced efficiency in mitochondrial energy production. By inhibiting NNMT, 5AM has been shown to:

Increase NAD+ availability, improving energy metabolism.
Reduce fat accumulation in animal models.
Improve insulin sensitivity and glucose handling.
Enhance overall mitochondrial function.

Research Applications

While still experimental, 5AM has drawn attention for its potential in multiple areas of metabolic research:

Obesity research: Animal studies suggest significant reductions in fat mass.
Metabolic syndrome: Investigated for improving insulin resistance and glucose tolerance.
Anti-aging research: By maintaining NAD+ levels, 5AM may support mitochondrial function and longevity studies.
Energy metabolism: Possible applications in sports science and endurance research.

Interesting Fact

NNMT inhibitors like 5AM are being studied not just for fat loss, but also for their potential role in extending healthy lifespan. NAD+ metabolism is a central focus of modern longevity research, and 5AM’s ability to boost NAD+ availability makes it a unique tool in this space.

Diet & Lifestyle Notes

Because 5AM research centers on metabolism and NAD+ pathways, lifestyle factors play an important role in results:

Diet quality: High-protein, whole-food diets support better metabolic health than processed or sugar-heavy diets.
Exercise: Resistance training and cardio both increase mitochondrial efficiency and may complement NAD+ research.
Hydration: Adequate water intake is essential for metabolic function and recovery.
Fasting: Some studies suggest intermittent fasting or calorie restriction enhances NAD+ metabolism, which may align with 5AM research goals.

Note: These are general wellness insights that may be useful in a research context. They are not medical advice.

Reconstitution & Handling

Reconstitute 5AM with sterile bacteriostatic water. Add water slowly to the vial, swirl gently until fully dissolved, and avoid shaking vigorously. Store reconstituted solution refrigerated at 2–8 °C.

Suggested Research Dosing

In preclinical models, 5AM has typically been studied at low daily doses, often administered for metabolic and fat loss endpoints. No standardized clinical dosing exists. Researchers are encouraged to begin conservatively and adjust based on experimental design.

To plan precise concentrations and draw volumes, use our Peptide Calculator.