Most peptides arrive as a white, lyophilized (freeze-dried) powder in a sealed vial. To use them in research, they must be reconstituted - dissolved in a sterile liquid such as Bacteriostatic Water (BAC Water). This guide walks you through the process step by step.
What You Need
- Lyophilized peptide vial
- Bacteriostatic Water (BAC Water)
- Alcohol swabs
- Insulin syringe (1 mL, 100 units)
- Sterile mixing syringe (3 mL or 5 mL)
Step-by-Step Reconstitution
- Clean the vial stoppers: Wipe both the peptide vial and the BAC water vial tops with an alcohol swab.
- Draw the BAC water: Using a sterile syringe, pull up the desired amount of BAC water (see below for how much).
- Inject slowly: Insert the needle into the peptide vial and let the water flow slowly down the inside wall — avoid spraying directly onto the powder.
- Swirl gently: Do not shake. Roll or swirl the vial until the powder fully dissolves. The solution should be clear, without flakes or cloudiness.
- Label the vial: Write the concentration (e.g., “5 mg/mL”), the date mixed, and storage instructions.
- Store correctly: Keep reconstituted peptides refrigerated (2–8 °C). Most remain stable for 20–30 days.
How Much Water Should I Add?
This depends on how concentrated you want your solution to be. A common starting point is 2 mL of BAC water into a 10 mg vial, which makes a concentration of 5 mg/mL. On a 100-unit insulin syringe, that equals 1 mg for every 20 units.
Instead of doing math by hand, use our Peptide Dose Calculator to instantly generate reconstitution ratios for any vial size and desired concentration.
Example - 10 mg Peptide Vial
| BAC Water Added | Final Concentration | Insulin Syringe Reading |
|---|---|---|
| 1 mL | 10 mg/mL | 1 mg = 10 units |
| 2 mL | 5 mg/mL | 1 mg = 20 units |
| 4 mL | 2.5 mg/mL | 1 mg = 40 units |
Tips for Best Results
- Always use BAC water instead of plain sterile water if you plan to store the peptide for more than one use.
- If the solution looks cloudy or has particles that won’t dissolve, discard it - this may indicate contamination or poor peptide quality.
- Write everything on the label (name, concentration, date) so there’s no confusion later.
Explore More
• What is BAC Water?
• Peptide Dose Calculator
• Medical Supplies Collection
Disclaimer: For laboratory research use only. Not for human consumption, therapeutic, or diagnostic use. Follow local laws and regulations.
